In order to filter out Gaussian noise and impulse noise at the same time, and get high resolution image in super-resolution reconstruction, a method with L1 and L2 mixed norm and Bilateral Total Variation (BTV) regularization was proposed for sequence images super-resolution. Firstly, multi-resolution optical flow model was used to register low-resolution sequence images and the registration precision was up to sub-pixel level, then the complementary information was used to raise image resolution. Secondly, taking advantage of L1 and L2 mixed norm, BTV regularization algorithm was used to solve the ill-posed problem. Lastly, the proposed algorithm was used to sequence images super-resolution. Experimental results show that the method can decrease the mean square error and increase Peak Signal-to-Noise Ratio (PSNR) by 1.2 dB to 5.2 dB. The algorithm can smooth Gaussian and impulse noise, protect image edge information and improve image identifiability, which provides good technique basis for license plate recognition, face recognition, video surveillance, etc.